Untargeted metabolomics of cell-free media from suspension-adapted cells producing adeno-associated viruses

Cell Therapy Catapult Ltd is seeking through this Open Tender Process to contract with a suitably capable Bidder to provide the Services for the untargeted metabolomics of cell-free media from suspension-adapted cells producing adeno-associated viral vectors (AAV). Briefly, human cells will be subjected to AAV production in parallel with controls. A maximum of 35 sample and two medium controls will be generated in this experiment, designated as "Run 1", and sent to the awarded Bidder each as technical replicates (maximum 3 per sample). The samples will then be processed by the awarded Bidder as per requirements detailed in Specification (Appendix B). In addition to "Run 1", two additional runs ("Run 2" and "Run 3") may be performed at our sole discretion. Importantly, the experimental setup for both "Run 2" and "Run 3" will be similar to that for "Run 1", but sample numbers may differ if additional conditions are included or excluded. A maximum of 37 samples (i.e., including medium controls) will be obtained for either "Run 2" and "Run 3". Subsequent runs or analysis may be performed, contingent on the results obtained from the 3 planned runs. This will be to ensure robustness of the findings or to meet unforeseen project needs. This will be based on satisfactory performance and continued need.