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AwardedFind a Tender · award

Advancing the metabarcoding assay validation framework

Buyer: Natural England →

BuyerNatural England
StatusAwarded
DeadlineNot published
ValueValue not published
Published11 Jul 2025

What is being bought

DNA-based methods have the potential to significantly change how we monitor and assess ecosystems. Natural England has been exploring the use of these methods for environmental monitoring for several years, delivering a series of reports which focus on the development of DNA-based methods with potential in a particular area. These methods are now being used more widely within Natural England, particularly the detection of ecological communities using environmental DNA (eDNA). This is often conducted by employing metabarcoding assays which target specific species assemblages (e.g. fish, mammals) or broader taxonomic groups (e.g. vertebrates, invertebrates, bacteria). The DNA Team aims to operationalise DNA-based methods and produces guidance for using them in monitoring projects. In order to provide sound recommendations, it is important that we understand the limitations of, and uncertainties with, DNA-based methods. Natural England staff need to be able to understand how DNA-derived data can be used, and the confidence they can have in data derived using different DNA-based methods which have been validated to different stages. Natural England previously commissioned the development of a framework to enable end users to assess confidence in metabarcoding assays and results (Pagaling and others, under review; a copy of the framework can be provided by Natural England upon request by emailing monitoring.innovation@naturalengland.org.uk). By using the checklist and levelled scale, end users can appropriately interpret results. It also provides validation and reporting standards for the development of new metabarcoding assays. The framework will enable end-users to determine the recommended scenarios for application of a given assay and improve assay performance with further validation. As part of development, the framework was trialled against five commonly used assays in the eDNA literature, with specific climate and habitat combinations to account for inherent differences in biodiversity between climates and different workflows used across habitats. Due to time and resource, only temperate climates and one or two habitats for each assay were considered, and the number of metabarcoding studies evaluated to assign a level for each assay varied. This project will advance the metabarcoding assay validation framework by expanding upon this trial to conduct a comprehensive literature review and meta-analysis, covering more assays being used for different purposes and in different contexts. Feedback on the framework will be sought from the wider eDNA community and a user guide created for applying the framework.

Lot details

Lot 1

No lot description published.

Statuscomplete

Comparable-procurement analytics

No usable CPV category has been published, so a comparable market set cannot yet be built.

Procurement strategy & market signals

Framework agreementNot published
Dynamic purchasing systemNot published
Competitive procurementNot published
Recurring requirementNot published
Procurement method rationaleNot published
Rationale classificationsNot published
Special regimeNot published
Covered byNot published
Submission policyNot published
Selection criteriaNot published
Risk detailsNot published

Planning & early market engagement

BudgetNot published
No-engagement rationaleNot published
Planning documents0
Planning milestones0

No planning milestones published.

Related procurements

No linked framework, prior procurement or reprocurement published.

Awards

Contracts

1

Statusactive
Value£41,574

Documents & submission route

No documents are published in the current source record.

Source data inventory

Diagnostic view. “Not published” means this current release does not provide a value.

OCIDocds-h6vhtk-0524a3
Latest release ID039190-2025
Latest release timestampFri Jul 11 2025 10:23:24 GMT+0000 (Coordinated Universal Time)
Sourcefind-a-tender
Official notice URLNot published
Tender statuscomplete
Procurement methodopen
Procurement method detailsBelow threshold - open competition
Main procurement categoryNot published
Above thresholdNot published
Legal basis2023/54
Tender period: startNot published
Tender period: endNot published
Expression of interest deadlineNot published
Enquiry deadlineNot published
Award period: startNot published
Award period: endNot published
Submission method detailsNot published
Submission languagesNot published
Electronic catalogue policyNot published
Total tender valueNot published
Tender lots in source1
Tender items in source0
Tender documents in source0
Awards in latest release1
Contracts in latest release1
Parties in latest release2

Notice history

DateEventReference
11 Jul 2025award, contract039190-2025
6 Jun 2025tenderUpdate030739-2025
4 Jun 2025tenderUpdate029886-2025
27 May 2025tenderUpdate027852-2025
23 May 2025tender027002-2025

All source data

Unmodified official OCDS data retained by Tenderline for this procurement process.

Complete current OCDS release JSON
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Complete JSON history (5 releases)
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4 Jun 2025 · 029886-2025 · tenderUpdate
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27 May 2025 · 027852-2025 · tenderUpdate
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23 May 2025 · 027002-2025 · tender
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