The Supply and Installation of a HIGH RESOLUTION FOURIER TRANSFORM BENCH-TOP LIQUID CHROMATROGRAPHY-ORBITRAP MASS SPECTROMETER (THERMO ORBITRAP EXPLORIS 240) to the University of Birmingham University of Birmingham Project Reference: SC10850/25

Total cost excluding VAT: £356,548.16 The equipment (Thermo Orbitrap Exploris 240) that we are requesting will permit us to detect changes in multiple metabolic pathways in a single analytical run as it combines the high mass resolution observed in 'multi-reflecting time of flight' instruments with increased sensitivity giving the ability to detect and gain additional molecular information on high numbers of metabolites present in low abundance as found in with 'triple quadrupole' instruments. Importantly for us (Metabolic Tracer Analysis Core (MTAC)), the high mass resolution will also allow us to deconvolute changes in mass due to incorporation of different stable isotopes (e.g. 15N, 13C, 2H), giving us the ability to examine nutrient use in more complex ways. This model is the only such instrument - the 'Orbitrap' part of the mass spectrometer is what provides this capability highlighted above, and Thermo Electron Manufacturing Limited trading as Thermo Fisher Scientific hold the patent for this technology. It includes nitrogen generator with high purity collision gas module, biocompatible liquid chromatography system, and full system integration with proprietary Thermo software (including tracing analysis software), all required to maintain the scientific edge of our metabolic research.